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E1611-94 Standard Guide for Conducting Sediment Toxicity Tests with Marine and Estuarine Polychaetous Annelids

Copyright 1997 AMERICAN SOCIETY FOR TESTING AND MATERIALS, West Conshohocken, PA. All rights reserved.

1. Scope

1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of potentially contaminated sediment, or of a test material added experimentally to contaminated or uncontaminated sediment, on marine or estuarine infaunal polychaetes during 10-day or 20 to 28-day exposures. These procedures are useful for testing the effects of various geochemical characteristics of sediments on marine and estuarine polychaetes and could be used to assess sediment toxicity to other infaunal taxa, although modifications of the procedures appropriate to the test species might be necessary. Procedures for the 10-day static test are described for Neanthes arenaceodentata and Neanthes virens and for the 20 to 28-day static-renewal sediment toxicity for N. arenaceodentata .

1.2 Modifications of these procedures might be appropriate for other sediment toxicity test procedures, such as flow-through or partial life-cycle tests. The methods outlined in this guide should also be useful for conducting sediment toxicity tests with other aquatic taxa, although modifications might be necessary. Other test organisms might include other species of polychaetes, crustaceans, and bivalves.

1.3 Other modifications of these procedures might be appropriate for special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparisons of the results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting sediment tests with infaunal organisms.

1.4 These procedures are applicable to sediments contaminated with most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. These procedures can be used with appropriate modifications to conduct sediment toxicity tests on factors such as temperature, salinity, dissolved oxygen (DO), and natural sediment characteristics (for example, particle size distribution, organic carbon content, and total solids). These procedures can also be used to conduct bioconcentration tests and in situ tests, and to assess the toxicity of potentially contaminated field sediments, or of materials such as sewage sludge, oils, particulate matter, and solutions of toxicants added to sediments. A median lethal concentration (LC50) or median sublethal effect concentration (EC50) of toxicants or of highly contaminated sediment mixed into uncontaminated sediment can be determined. Materials adhering to sediment particles or dissolved in interstitial water can be tested.

1.5 The results of 10-day toxicity tests with contaminated sediments can be reported as a LC50 if a series of concentrations is tested or as a percent mortality relative to a control or reference sediment. The results of 20 to 28-day toxicity tests with contaminated sediments can be reported as a LC50 if a series of concentrations is tested or as a percent mortality or growth relative to a control or reference sediment.

1.6 This guide is arranged as follows:

Section Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Interferences 6 Apparatus 7 Facilities 7.1 Construction Materials 7.2 Test Chambers 7.3 Cleaning 7.4 Acceptability 7.5 Hazards 8 Test Water 9 General Requirements 9.1 Source 9.2 Preparation 9.3 Characterization 9.4 Test and Control Sediments 10 General 10.1 Characterization 10.2 Control Sediment 10.3 Field-Collected Test Sediment 10.4 Reference Sediment 10.5 Laboratory-Spiked Test Sediment 10.6 Test Concentration(1) 10.7 Addition of Toxicant to Sediment 10.8 Test Organism: 11 Species 11.1 Age 11.2 Feeding 11.3 Source 11.4 Collection and Handling 11.5 Quality 11.6 Experimental Design 12 Controls 12.2 Field Survey Design 12.3 Laboratory Experiments 12.4 Procedure 13 Dissolved Oxygen 13.1 Temperature 13.2 Salinity 13.3 Light 13.4 Feeding 13.5 Beginning of Test 13.6 Duration of Test 13.7 Biological Data 13.8 Other Measurements 13.9 Analytical Methodology 14 Acceptability of Test 15 Interpretation of Results 16 Report 17 Keywords 18 Annexes Neanthes arenaceodentata A1 Neanthes virens A2

1.7 The values stated in SI units are to be regarded as the standard.

1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazards statements are given in Section 8.

 
 
 
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