Note: This web page is a part of DTSC's Hazardous Waste Classification training course.
Acute Aquatic Toxicity
Aquatic toxicity is measured by fish bioassay. A waste can be tested for this criterion by placing some waste in a test water tank and introducing one of three acceptable fish species: fathead minnows, rainbow trout or golden shiners.
Waste is hazardous by aquatic toxicity1 if a 96-hour LC50 is less than 500 mg/liter.
96-hour LC50 < 500 mg/l = acute aquatic toxicity
For more information, here is the procedure for the acute aquatic toxicity bioassay per 22 CCR section 66261.24(a)(6).
References
1 22 CCR Section 66261.24(a)(6)
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