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BiMelyze Field Screening Assay-Certification No: 95-01-014

Final Decision to Certify Hazardous Waste Environmental Technology

Technology Description:

The BiMelyze Field Screening Assay (“Tube Assay”) test kit provides the user with a semi-quantitative immunoassay system for the detection of mercury in water and a Soil Extraction Kit which is used together with strong acid reagents, which the user provides, for the conversion of other forms of mercury into mercuric ion and subsequent processing by the Tube Assay. Mercuric ion is the chemical form of mercury detected by the immunoassay. Of the inorganic and organic forms of mercury tested, only methyl mercury requires an extended extraction step for detection by the assay. Provided the assay is used properly, detection of mercury is possible in a range of 0.25 to 25 ppb in water, 0.5 to 50 ppm in soil. Actual use is for classification of samples above and below the target levels of the actual testing kit provided by the manufacturer, typically 5 and 15 ppm for soil; for water, a 100 ppm standard is provided for dilution by the user as appropriate for the study. Readings about these target levels are obtained by comparing sample and standard either visually or in a battery-powered differential photometer supplied by the manufacturer. For field studies in a higher concentration range, the user should rely on the manufacturer to provide a customized assay kit for that range or use the manufacturer’s dilution buffer, as appropriate. DTSC’s findings are described in greater detail in an evaluation report.

Issued to:
  BioNebraska, Inc.
  Lincoln, NE
  Dr. Larry Carlson
  (402) 470-2100
Effective Date:
  August 21, 1995
Expiration Date:
  August 21, 1998
DTSC Contact:
  Dr. Wolfgang Fuhs
  (510) 540-3076

A copy of the published Certification Notice may be obtained by contacting us at:


Department of Toxic Substances Control
  Office of Pollution Prevention and Technology Development
  P.O. Box 806
  Sacramento, California 95812-0806
  Phone: (916) 322-3670
  Fax: (916) 327-4494

File last updated: October 9, 1996