Website Archive

Content on DTSC’s Website Archive is no longer being updated. It is being provided for informational purposes only and may be out of date, contain technical inaccuracies, typographical errors or broken links. This content is not promised or guaranteed to be correct, current or complete. DTSC assumes no responsibility (and expressly disclaims responsibility) for updating this site. Any reliance on the content contained herein is at the user’s own risk. Users are solely responsible for confirming the accuracy and completeness of all posted information before citing or using the information. By accessing this site, users agree that DTSC shall not be held liable for any claim, loss or damages which may result from the use of, access to, or inability to use the content contained herein.

EnviroGard TNT in Soil Test Kit-(Trinitrotoluene in Soil)-Certification No: 95-01-016

Final Decision to Certify Hazardous Waste Environmental Technology

Technology Description:

The EnviroGard TNT assay system uses enzyme-linked immunosorbent assay (ELISA) technology. It differs from other systems in that polyclonal antibodies are attached to test tubes. The system uses soil extraction with acetone. The immunoassay system is semi-quantitative. Provided that the materials are used properly, detection is possible at 0.2 to 15.0 ppm in soil; ranges can be extended upward by dilution of the extracts. Quantitative readings about a selected target level are obtained in a microprocessor- equipped, hand-held, battery-operated photometer or a compact table top photometer supplied by the manufacturer, or a standard laboratory photometer. The calibration is not biased, but the user is instructed to select target levels so as to determine the degree of confidence in avoiding false negative results. The reaction with other nitroaromatics is insignificant except for 1,3,5-trinitrobenzene (a contaminant in technical grade TNT), HMX, RDX, Dinoseb, 2,4-dinitrophenol, and the mononitroaromatics do not interfere. Differences in the extraction efficiency of TNT from various soils may cause positive or negative errors in the immunoassay results. Users should evaluate these factors before attempting to quantify results.

Great care is required and established safety measures must be observed in sampling and handling soil containing explosives. As with similar assays, certain temperature controls are required for reagent storage and for carrying out the assay. The assay should be used only by trained individuals to reduce operator-caused variability.

Issued to:
Millipore, Inc.
80 Ashby Road
Bedford, MA 01730
EnSys, Inc.
P.O. Box 14063
Research Triangle
Park, NC 27709
(919) 941-5509
EnSys, Inc.
432 Villa Point Drive
Newport Beach, CA 92660
Dwight Denham
Southwest Regional Manager
(800) 242-7472, Ext. 173
(714) 644-8650
Effective Date:
March 11, 1996
Expiration Date:
March 11, 1999
DTSC Contact:
Dr. Wolfgang Fuhs
(510) 540-3076

A copy of the published Certification Notice may be obtained by contacting us at:

Department of Toxic Substances Control
Office of Pollution Prevention and Technology Development
P.O. Box 806
Sacramento, California 95812-0806
Phone: (916) 322-3670
Fax: (916) 327-4494

File last updated: October 21, 1996